1) ABSTRACT The purpose of this sample was to carry forth the build of enzyme concentration, temperature, pH level, and an inhibitor on a type of enzyme called Perixodase. The purpose of the audition was also to understand the relationship between the change in absorbance with time and the enzyme reply direct. To perform the experiment, the class was divided into groups. apiece group took on one of the factors affecting the enzyme. Before radical the actual experiment, we apply the mixing tables that were given to us to situate up the test tubes with the appropriate substances. We followed the same procedure for singly experiment variable (1-5). We then rid the cell of toxic hydrogen peroxide (H2O2) so the cell would not make damaged, as stated in our lab manual. For every empirical variable, test tube 1 was always the control. Once the solutions were pose we mixed them and poured them into the cuvette and use the spectrophotometer machine to run the enzyme and determine its site and its absorbance. When the actual experiment was done we all shared the results and graphed them. 2) entering Before beginning the actual experiment we had to become well-known(prenominal) with a spectrometer machine and its functions.
It is basically an instrument used to measure the amount of light absorbed by a solution (Frankhauser, David B). As stated in our lab manual, the darker the solution, the greater its absorbance. The reason why we used this machine was to figure divulge the relationship between the change in absorbance with time and the enzyme reaction rate. The e nzyme reaction rate was determined when plo! t of grounds of absorbance versus time were constructed. The slope of all(prenominal) plot was related to the reaction rate. After running a little experiment to learn how to use the spectrophotometer, we learned or so the background information of enzymes. Enzymes are proteins that serve as catalyst (Ophardt, Charles E.). By definition, a catalyst is a substance that initiates or accelerates...If you want to get a full essay, order it on our website: OrderCustomPaper.com
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